Back to Search
Start Over
Neuroprotective Effects of Sigesbeckia pubescens Extract on Glutamate-Induced Oxidative Stress in HT22 Cells via Downregulation of MAPK/caspase-3 Pathways.
- Source :
-
Cellular and molecular neurobiology [Cell Mol Neurobiol] 2018 Mar; Vol. 38 (2), pp. 497-505. Date of Electronic Publication: 2017 May 05. - Publication Year :
- 2018
-
Abstract
- Sigesbeckia pubescens (SP) is a traditional Chinese medicine, possessing antioxidant and anti-inflammatory activities. In this study, we evaluate the neuroprotective activities of SP extract on glutamate-induced oxidative stress in HT22 cells and the molecular mechanism underlying neuroprotection. We applied 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), crystal violet, reactive oxygen species (ROS), lactate dehydrogenase (LDH), quantitative real-time polymerase chain reaction (qPCR), and western blot analyses for assessing the neuroprotective effects of SP extract. The experimental study revealed that SP considerably increased the cell viability, and reduced the oxidative stress promoted ROS and LDH generation in HT22 cells in a dose-dependent manner. Additionally, the morphology of HT22 cells was effectively improved by SP. Upregulated gene expressions of mitogen-activated protein kinase (MAPK) were markedly attenuated by SP. Similarly, SP notably suppressed the ROS-mediated phosphorylation of MAPK (pERK1/2, pJNK, and pp38) cascades and activation of apoptotic factor caspase-3 signaling pathway that overall contributed to the neuroprotection. Taken together, SP may exert neuroprotective effects via alteration of MAPK and caspase-3 pathways under oxidative stress condition. Therefore, SP is a potential agent for preventing oxidative stress-mediated neuronal cell death.
- Subjects :
- Animals
Apoptosis drug effects
Apoptosis physiology
Cell Line
Cell Survival drug effects
Cell Survival physiology
Dose-Response Relationship, Drug
Down-Regulation drug effects
Down-Regulation physiology
Drugs, Chinese Herbal isolation & purification
MAP Kinase Signaling System physiology
Mice
Neuroprotective Agents isolation & purification
Oxidative Stress physiology
Caspase 3 metabolism
Drugs, Chinese Herbal pharmacology
Glutamic Acid toxicity
MAP Kinase Signaling System drug effects
Neuroprotective Agents pharmacology
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6830
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cellular and molecular neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 28477054
- Full Text :
- https://doi.org/10.1007/s10571-017-0496-y