Back to Search
Start Over
Purification and characterization of recombinant human bile salt-stimulated lipase expressed in milk of transgenic cloned cows.
- Source :
-
PloS one [PLoS One] 2017 May 05; Vol. 12 (5), pp. e0176864. Date of Electronic Publication: 2017 May 05 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and from the lactating mammary gland into the milk of some species, notably humans but not cows. BSSL in breast milk facilitates digestion and absorption of milk fat and promotes growth of small for gestational age preterm infants. Thus, purified recombinant human BSSL (rhBSSL) can be used for treatment of patients with fat malabsorption and expressing rhBSSL in the milk of transgenic cloned cows would therefore be a mean to meet a medical need. In the present study, a vector pBAC-hLF-hBSSL was constructed, which efficiently expressed active rhBSSL in milk of transgenic cloned cows to a concentration of 9.8 mg/ml. The rhBSSL purified from cow milk had the same enzymatic activity, N-terminal amino acid sequence, amino acid composition and isoelectric point and similar physicochemical characteristics as human native BSSL. Our study supports the use of transgenic cattle for the cost-competitive, large-scale production of therapeutic rhBSSL.
- Subjects :
- Animals
Animals, Genetically Modified
Blotting, Western
Cattle
Cloning, Organism
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Genetic Vectors
Hot Temperature
Humans
Hydrogen-Ion Concentration
Kinetics
Lipase chemistry
Lipase genetics
Lipase therapeutic use
Malabsorption Syndromes drug therapy
Polymerase Chain Reaction
Lipase isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 28475629
- Full Text :
- https://doi.org/10.1371/journal.pone.0176864