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Evaluation of plant-produced Clostridium perfringens type D epsilon toxoid in a vaccine against enterotoxaemia in sheep.

Authors :
Mokoena T
Chakauya E
Crampton M
Weyers B
Tselanyane M
Tsekoa T
Chikwamba R
Source :
The Onderstepoort journal of veterinary research [Onderstepoort J Vet Res] 2017 Apr 25; Vol. 84 (1), pp. e1-e7. Date of Electronic Publication: 2017 Apr 25.
Publication Year :
2017

Abstract

Enterotoxaemia (pulpy kidney) is a common bacterial disease of sheep caused by Clostridium perfringens type D epsilon toxin. It has mortality rates of up to 30% in non-vaccinated animals. Current vaccines from whole cell cultures are expensive to manufacture and can induce local inflammatory responses in sheep. They usually have reduced immunogenicity because of the difficulty of standardising the inactivation step in vaccine manufacturing. In the current study, we evaluated the safety and potency of a recombinant plant-made epsilon toxoid protein (r-Etox) as an affordable and safer alternative vaccine for developing countries. Results of injection site reactions, rectal temperature and toxin neutralisation test in single and prime- boost inoculations of mice, guinea pigs and sheep suggest that the product is not toxic to animals and could protect sheep against enterotoxaemia.

Details

Language :
English
ISSN :
2219-0635
Volume :
84
Issue :
1
Database :
MEDLINE
Journal :
The Onderstepoort journal of veterinary research
Publication Type :
Academic Journal
Accession number :
28470084
Full Text :
https://doi.org/10.4102/ojvr.v84i1.1271