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Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice.
- Source :
-
Indian journal of pharmacology [Indian J Pharmacol] 2017 Jan-Feb; Vol. 49 (1), pp. 34-41. - Publication Year :
- 2017
-
Abstract
- Objective: The objective of this study was to study the effect of ethanol extract of Clerodendrum serratum (EECS) Linn. on acute restraint stress (ARS)-induced depressive-like behavior and biochemical alterations in mice.<br />Materials and Methods: Ethyl acetate and n-butanol fractions of EECS were analytically characterized for the flavonoid components, apigenin (API) and luteolin (LUT) by reverse-phase high-performance liquid chromatography. Behavioral tests, namely, forced-swim test and tail-suspension test were performed for assessing antidepressant-like effect and anxiolytic activity in mice. Oxidative stress parameters and biochemical alterations in mice brain tissue were also performed.<br />Statistical Analysis: Expression of data was done as mean ± standard error of mean. The normally distributed data were subjected to two-way ANOVA followed by Dunnett's test. P < 0.05 was considered statistically significant.<br />Results: The study showed that flavonoids, API and LUT were present in ethyl acetate and n-butanol fractions of EECS, which significantly reversed ARS-induced depressive-like behavior without affecting locomotion. EECS also attenuated oxidative damage caused by ARS. The level of norepinephrine and 5-hydroxytryptamine was also significantly restored by pretreatment with EECS for 7 days.<br />Conclusion: EECS significantly alleviated ARS-induced depressive-like behavior without affecting locomotion.<br />Competing Interests: There are no conflicts of interest.
- Subjects :
- Animals
Brain metabolism
Chromatography, High Pressure Liquid
Depression drug therapy
Disease Models, Animal
Hindlimb Suspension
Male
Mice
Motor Activity drug effects
Neuroprotective Agents isolation & purification
Oxidative Stress drug effects
Plant Leaves
Restraint, Physical
Serotonin metabolism
Stress, Psychological drug therapy
Swimming
Antidepressive Agents pharmacology
Clerodendrum chemistry
Neuroprotective Agents pharmacology
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1998-3751
- Volume :
- 49
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Indian journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 28458420
- Full Text :
- https://doi.org/10.4103/0253-7613.201028