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Live imaging of primary ocular vasculature formation in zebrafish.
- Source :
-
PloS one [PLoS One] 2017 Apr 26; Vol. 12 (4), pp. e0176456. Date of Electronic Publication: 2017 Apr 26 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- Ocular vasculature consists of the central retinal and ciliary vascular systems, which are essential to maintaining visual function. Many researchers have attempted to determine their origins and development; however, the detailed, stepwise process of ocular vasculature formation has not been established. In zebrafish, two angioblast clusters, the rostral and midbrain organizing centers, form almost all of the cranial vasculature, including the ocular vasculature, and these are from where the cerebral arterial and venous angioblast clusters, respectively, differentiate. In this study, we first determined the anatomical architecture of the primary ocular vasculature and then followed its path from the two cerebral angioblast clusters using a time-lapse analysis of living Tg(flk1:EGFP)k7 zebrafish embryos, in which the endothelial cells specifically expressed enhanced green fluorescent protein. We succeeded in capturing images of the primary ocular vasculature formation and were able to determine the origin of each ocular vessel. In zebrafish, the hyaloid and ciliary arterial systems first organized independently, and then anastomosed via the inner optic circle on the surface of the lens by the lateral transfer of the optic vein. Finally, the choroidal vascular plexus formed around the eyeball to complete the primary ocular vasculature formation. To our knowledge, this study is the first to report successful capture of circular integration of the optic artery and vein, lateral transfer of the optic vein to integrate the hyaloidal and superficial ocular vasculatures, and formation of the choroidal vascular plexus. Furthermore, this new morphological information enables us to assess the entire process of the primary ocular vasculature formation, which will be useful for its precise understanding.
- Subjects :
- Animals
Animals, Genetically Modified metabolism
Embryo, Nonmammalian metabolism
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Imaging, Three-Dimensional
In Situ Hybridization
Microscopy, Fluorescence, Multiphoton
Retinal Artery pathology
Retinal Artery physiology
Retinal Vein pathology
Retinal Vein physiology
Zebrafish growth & development
Retinal Artery anatomy & histology
Retinal Vein anatomy & histology
Time-Lapse Imaging
Zebrafish physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 28445524
- Full Text :
- https://doi.org/10.1371/journal.pone.0176456