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Identification of HDA15-PIF1 as a key repression module directing the transcriptional network of seed germination in the dark.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2017 Jul 07; Vol. 45 (12), pp. 7137-7150. - Publication Year :
- 2017
-
Abstract
- Light is a major external factor in regulating seed germination. Photoreceptor phytochrome B (PHYB) plays a predominant role in promoting seed germination in the initial phase after imbibition, partially by repressing phytochrome-interacting factor1 (PIF1). However, the mechanism underlying the PHYB-PIF1-mediated transcription regulation remains largely unclear. Here, we identified that histone deacetylase15 (HDA15) is a negative component of PHYB-dependent seed germination. Overexpression of HDA15 in Arabidopsis inhibits PHYB-dependent seed germination, whereas loss of function of HDA15 increases PHYB-dependent seed germination. Genetic evidence indicated that HDA15 acts downstream of PHYB and represses seed germination dependent on PIF1. Furthermore, HDA15 interacts with PIF1 both in vitro and in vivo. Genome-wide transcriptome analysis revealed that HDA15 and PIF1 co-regulate the transcription of the light-responsive genes involved in multiple hormonal signaling pathways and cellular processes in germinating seeds in the dark. In addition, PIF1 recruits HDA15 to the promoter regions of target genes and represses their expression by decreasing the histone H3 acetylation levels in the dark. Taken together, our analysis uncovered the role of histone deacetylation in the light-regulated seed germination process and identified that HDA15-PIF1 acts as a key repression module directing the transcription network of seed germination.<br /> (© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Acetylation
Arabidopsis growth & development
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Basic Helix-Loop-Helix Transcription Factors metabolism
Darkness
Gene Expression Regulation, Developmental
Germination genetics
Histone Deacetylases metabolism
Histones genetics
Histones metabolism
Light Signal Transduction
Phytochrome B metabolism
Protein Processing, Post-Translational
Seeds growth & development
Seeds metabolism
Transcription, Genetic
Arabidopsis genetics
Arabidopsis Proteins genetics
Basic Helix-Loop-Helix Transcription Factors genetics
Gene Expression Regulation, Plant
Genome, Plant
Histone Deacetylases genetics
Phytochrome B genetics
Seeds genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 45
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 28444370
- Full Text :
- https://doi.org/10.1093/nar/gkx283