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Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing.
- Source :
-
Journal of periodontology [J Periodontol] 2017 Aug; Vol. 88 (8), pp. 799-807. Date of Electronic Publication: 2017 Apr 25. - Publication Year :
- 2017
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Abstract
- Background: Loss of alveolar ridge width and height after tooth extraction is well documented, but models to evaluate ridge preservation are neither standardized nor cost-effective. This rat model characterizes the pattern of bone turnover and inflammation after extraction and bone grafting with or without local simvastatin (SIM).<br />Methods: Fifty retired-breeder rats underwent extraction of the maxillary right first molar and standard surgical defect creation under inhalation/local anesthesia. The left side of each animal served as unmanipulated control. Untreated groups (n = 8 to 9 per group) were compared (analysis of variance, t test) at days 0, 7, 14, and 28 for alveolar ridge height and width and for markers of inflammation and bone turnover by microcomputed tomography, histology, and enzyme-linked immunosorbent assay. Seventeen additional specimens had defects grafted with either bone mineralized matrix (BMM) or a BMM+SIM conjugate.<br />Results: Extraction-induced bone loss (BL) was noted on buccal, palatal, and interproximal height (P <0.05) and ridge width (P <0.01). Week 1 inflammation positively correlated with ridge height; thereafter, a more intense inflammatory reaction corresponded to reduction in alveolar bone height and density (r = 0.74; P <0.05; Spearman). BMM+SIM preserved the most interproximal bone height (P <0.01), increased ridge width and bone density (P <0.01), enhanced 7-day prostaglandin E2 (P <0.01), and reduced 28-day inflammation density (P <0.05).<br />Conclusions: The standard defect used in the current study paralleled human postextraction alveolar BL. Defect grafting, especially BMM+SIM, reduced inflammation and preserved bone.
- Subjects :
- Alveolar Bone Loss diagnostic imaging
Animals
Bone Remodeling physiology
Bone Transplantation methods
Enzyme-Linked Immunosorbent Assay
Maxilla surgery
Molar surgery
Rats
X-Ray Microtomography
Alveolar Bone Loss physiopathology
Alveolar Bone Loss therapy
Disease Models, Animal
Simvastatin pharmacology
Tooth Extraction
Tooth Socket surgery
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1943-3670
- Volume :
- 88
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 28440741
- Full Text :
- https://doi.org/10.1902/jop.2017.160771