Back to Search
Start Over
Characterization of a sulfated galactoglucan from Antrodia cinnamomea and its anticancer mechanism via TGFβ/FAK/Slug axis suppression.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2017 Jul 01; Vol. 167, pp. 229-239. Date of Electronic Publication: 2017 Mar 06. - Publication Year :
- 2017
-
Abstract
- A sulfated 1,4-β-d-galactoglucan (B86-III) with 1,6-branches was isolated and identified from Antrodia cinnamomea. The repeating unit of B86-III was proposed based on one-dimensional 1D ( <superscript>1</superscript> H, <superscript>13</superscript> C and DEPT-135) and 2D (DQF-COSY, TOCSY, HSQC and HMBC) NMR spectra. The conformation of the sugars was hypothesized to be a rare boat form instead of a <superscript>4</superscript> C <subscript>1</subscript> chair form. The sulfate substitutions were suggested to be on the C-2 and C-3 positions, resulting in the following structure: B86-III inhibited the viability of H1975 lung cancer cells via cell apoptosis, including the activation of caspase 3 and PARP. Transforming growth factor β receptor (TGFR) and its downstream signaling FAK and Slug are involved in lung tumorigenesis. B86-III downregulated TGFR I protein levels and inhibited FAK phosphorylation, resulting in inhibition of Slug expression and migration. This study is the first to characterize sulfated polysaccharides with a rare boat-form conformation and identify the mechanism of inhibition lung cancer cell.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Apoptosis
Cell Line, Tumor
Cell Survival
Humans
Lung Neoplasms pathology
Signal Transduction
Antineoplastic Agents chemistry
Antrodia chemistry
Focal Adhesion Kinase 1 metabolism
Galactans chemistry
Glucans chemistry
Polysaccharides, Bacterial chemistry
Snail Family Transcription Factors metabolism
Transforming Growth Factor beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 167
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 28433158
- Full Text :
- https://doi.org/10.1016/j.carbpol.2017.02.104