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Complementary approaches for the evaluation of biocompatibility of 90 Y-labeled superparamagnetic citric acid (Fe,Er) 3 O 4 coated nanoparticles.
- Source :
-
Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2017 Jun 01; Vol. 75, pp. 157-164. Date of Electronic Publication: 2017 Feb 10. - Publication Year :
- 2017
-
Abstract
- Magnetic nanoparticles (MNPs) are of immense interest for diagnostic and therapeutic applications in medicine. Design and development of new iron oxide-based MNPs for such applications is of rather limited breadth without reliable and sensitive methods to determine their levels in body tissues. Commonly used methods, such as ICP, are quite problematic, due to the inability to decipher the origin of the detected iron, i.e. whether it originates from the MNPs or endogenous from tissues and bodily fluids. One of the approaches to overcome this problem and to increase reliability of tracing MNPs is to partially substitute iron ions in the MNPs with Er. Here, we report on the development of citric acid coated (Fe,Er) <subscript>3</subscript> O <subscript>4</subscript> nanoparticles and characterization of their physico-chemical and biological properties by utilization of various complementary approaches. The synthesized MNPs had a narrow (6-7nm) size distribution, as consistently seen in atomic pair distribution function, transmission electron microscopy, and DC magnetization measurements. The particles were found to be superparamagnetic, with a pronounced maximum in measured zero-field cooled magnetization at around 90K. Reduction in saturation magnetization due to incorporation of 1.7% Er <superscript>3+</superscript> into the Fe <subscript>3</subscript> O <subscript>4</subscript> matrix was clearly observed. From the biological standpoint, citric acid coated (Fe,Er) <subscript>3</subscript> O <subscript>4</subscript> NPs were found to induce low toxicity both in human cell fibroblasts and in zebrafish (Danio rerio) embryos. Biodistribution pattern of the MNPs after intravenous administration in healthy Wistar rats was followed by the radiotracer method, revealing that <superscript>90</superscript> Y-labeled MNPs were predominantly found in liver (75.33% ID), followed by lungs (16.70% ID) and spleen (2.83% ID). Quantitative agreement with these observations was obtained by ICP-MS elemental analysis using Er as the detected tracer. Based on the favorable physical, chemical and biological characteristics, citric acid coated (Fe,Er) <subscript>3</subscript> O <subscript>4</subscript> MNPs could be further considered for the potential application as a diagnostic and/or therapeutic agent. This work also demonstrates that combined application of these techniques is a promising tool for studies of pharmacokinetics of the new MNPs in complex biological systems.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Line
Fibroblasts cytology
Humans
Magnetic Fields
Citric Acid chemistry
Citric Acid pharmacology
Coated Materials, Biocompatible chemistry
Coated Materials, Biocompatible pharmacokinetics
Europium chemistry
Europium pharmacology
Ferric Compounds chemistry
Ferric Compounds pharmacology
Fibroblasts metabolism
Materials Testing
Nanoparticles chemistry
Yttrium Radioisotopes
Zebrafish metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0191
- Volume :
- 75
- Database :
- MEDLINE
- Journal :
- Materials science & engineering. C, Materials for biological applications
- Publication Type :
- Academic Journal
- Accession number :
- 28415449
- Full Text :
- https://doi.org/10.1016/j.msec.2017.02.023