NANOG gene expression is regulated by the ETS transcription factor ETV4 in human embryonic carcinoma NCCIT cells.

Authors :: Park SW
Do HJ
Choi W
Song H
Chung HJ
Kim JH
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2017 Jun 03; Vol. 487 (3), pp. 532-538. Date of Electronic Publication: 2017 Apr 13.
Publication Year :: 2017
Abstract: We demonstrated that ETV4 is a transcriptional activator of the NANOG gene in human embryonic carcinoma NCCIT cells. The endogenous expression of NANOG and ETV4 in naïve cells was significantly down-regulated upon differentiation and by shRNA-mediated knockdown of ETV4. NANOG transcription was significantly upregulated by ETV4 overexpression. A putative ETS binding site (EBS) is present in the region (-285 to -138) of the proximal promoter. Site-directed mutagenesis of the putative EBS ( <superscript>-196</superscript> AGGATT <superscript>-191</superscript> ) abolished NANOG promoter activity and ETV4 interacted with this putative EBS both in vivo and in vitro. Our data provide the molecular details of ETV4-mediated NANOG gene expression.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)

Subjects :: Humans
Nanog Homeobox Protein metabolism
Proto-Oncogene Proteins c-ets
Tumor Cells, Cultured
Adenovirus E1A Proteins metabolism
Embryonal Carcinoma Stem Cells metabolism
Gene Expression Regulation, Neoplastic
Nanog Homeobox Protein genetics
Proto-Oncogene Proteins metabolism

Full Text Access

Tools