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Comparative diagnostic efficacy of recombinant LLO and PI-PLC-based ELISAs for detection of listeriosis in animals.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2017 Jun; Vol. 137, pp. 40-45. Date of Electronic Publication: 2017 Apr 12. - Publication Year :
- 2017
-
Abstract
- The present study for the first time evaluates the serodiagnostic efficacy of two recombinant antigens namely, listeriolysin O (rLLO) and phosphatidyl-inositol phospholipase C (rPI-PLC). Indirect ELISA with the above recombinant antigens was used on samples collected from bovines (n=106), goats (n=138) and pigs (n=92) having either a history of abortion, emaciation and/or apparently healthy animals. Isolation of Listeria was attempted from the blood samples using USDA-FSIS method. On screening of test sera by rLLO-based ELISA, antibodies against anti-listeriolysin O (ALLO) were observed in goats (22.46%), bovines (15.10%) and pigs (16.31%). As advocated, after adsorption of positive serum samples with streptolysin O (SLO), the seropositivity for ALLO was marginally reduced (p>0.05) in goats (21.73%) and bovines (10.38%), whereas, in pigs the reduction (5.43%) was significant (p<0.05). On the contrary, rPI-PLC-based ELISA revealed higher non-specific seropositivity for antilisterial antibodies in goats (45.65%), bovines (31.13%) and pigs (8.69%). Further, on comparing the seropositivity with isolation rate, of the 16 animals that were culturally-positive for L. monocytogenes, 15 showed ALLO positivity in unadsorbed as well as SLO-adsorbed sera by rLLO-based ELISA, however, rPI-PLC-based ELISA could detect seropositivity in only 5 animals. Moreover, rPI-PLC-based ELISA also showed seropositivity in those animals (7/30) that were culturally positive for other Listeria spp. In conclusion, rLLO can serve as a better antigen than rPI-PLC in ELISA for the serodiagnosis of listeriosis in animals; however, prior adsorption of test sera with SLO is required to avoid false positive results.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animal Diseases blood
Animal Diseases diagnosis
Animals
Antibodies, Bacterial blood
Antigens, Bacterial genetics
Bacterial Proteins blood
Bacterial Toxins genetics
Bacterial Toxins immunology
Cattle
Enzyme-Linked Immunosorbent Assay methods
Goats
Heat-Shock Proteins genetics
Heat-Shock Proteins immunology
Hemolysin Proteins genetics
Hemolysin Proteins immunology
Listeria enzymology
Listeria isolation & purification
Listeriosis blood
Listeriosis diagnosis
Listeriosis immunology
Phosphoinositide Phospholipase C genetics
Phosphoinositide Phospholipase C immunology
Recombinant Proteins genetics
Recombinant Proteins immunology
Sensitivity and Specificity
Serologic Tests methods
Serologic Tests veterinary
Streptolysins blood
Swine
Animal Diseases microbiology
Bacterial Toxins analysis
Enzyme-Linked Immunosorbent Assay veterinary
Heat-Shock Proteins analysis
Hemolysin Proteins analysis
Listeriosis veterinary
Phosphoinositide Phospholipase C analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 137
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 28411150
- Full Text :
- https://doi.org/10.1016/j.mimet.2017.04.005