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Hydrogen-rich saline attenuates spinal cord hemisection-induced testicular injury in rats.
- Source :
-
Oncotarget [Oncotarget] 2017 Jun 27; Vol. 8 (26), pp. 42314-42331. - Publication Year :
- 2017
-
Abstract
- To study how hydrogen-rich saline (HS) promotes the recovery of testicular biological function in a hemi-sectioned spinal cord injury (hSCI) rat model, a right hemisection was performed at the T11-T12 of the spinal cord in Wistar rats. Animals were divided into four groups: normal group; vehicle group: sham-operated rats administered saline; hSCI group: subjected to hSCI and administered saline; HRST group: subjected to hSCI and administered HS. Hind limb neurological function, testis index, testicular morphology, mean seminiferous tubular diameter (MSTD) and seminiferous epithelial thickness (MSET), the expression of heme oxygenase-1 (HO-1), mitofusin-2 (MFN-2), and high-mobility group box 1 (HMGB-1), cell ultrastructure, and apoptosis of spermatogenic cells were studied. The results indicated that hSCI significantly decreased the hind limb neurological function, testis index, MSTD, and MSET, and induced severe testicular morphological injury. The MFN-2 level was decreased, and HO-1 and HMGB-1 were overexpressed in testicular tissues. In addition, hSCI accelerated the apoptosis of spermatogenic cells and the ultrastructural damage of cells in the hypophysis and testis. After HS administration, all these parameters were considerably improved, and the characteristics of hSCI testes were similar to those of normal control testes. Taken together, HS administration can promote the recovery of testicular biological function by anti-oxidative, anti-inflammatory, and anti-apoptotic action. More importantly, HS can inhibit the hSCI-induced ultrastructural changes in gonadotrophs, ameliorate the abnormal regulation of the hypothalamic-pituitary-testis axis, and thereby promote the recovery of testicular injury. HS administration also inhibited the hSCI-induced ultrastructural changes in testicular spermatogenic cells, Sertoli cells and interstitial cells.
- Subjects :
- Animals
Apoptosis drug effects
Biomarkers
Disease Models, Animal
GTP Phosphohydrolases
Gene Expression
Germ Cells drug effects
Germ Cells metabolism
Heme Oxygenase-1 genetics
Heme Oxygenase-1 metabolism
Male
Membrane Proteins genetics
Membrane Proteins metabolism
Mitochondrial Proteins genetics
Mitochondrial Proteins metabolism
Neurological Rehabilitation
Pituitary Gland drug effects
Pituitary Gland ultrastructure
Rats
Recovery of Function drug effects
Sertoli Cells drug effects
Sertoli Cells metabolism
Sertoli Cells ultrastructure
Spinal Cord Injuries diagnosis
Testicular Diseases drug therapy
Testicular Diseases metabolism
Testis drug effects
Testis metabolism
Testis physiopathology
Testis ultrastructure
Hydrogen administration & dosage
Saline Waters
Spinal Cord Injuries complications
Testicular Diseases etiology
Testicular Diseases rehabilitation
Subjects
Details
- Language :
- English
- ISSN :
- 1949-2553
- Volume :
- 8
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Oncotarget
- Publication Type :
- Academic Journal
- Accession number :
- 28404953
- Full Text :
- https://doi.org/10.18632/oncotarget.15876