Back to Search
Start Over
Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase.
- Source :
-
Gene [Gene] 1988 May 15; Vol. 65 (1), pp. 1-11. - Publication Year :
- 1988
-
Abstract
- We have isolated a full-length human fetal muscle cDNA clone specifying the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme, cytochrome c oxidase (COX; EC 1.9.3.1), and a partial-length brain cDNA clone specifying the analogous bovine subunit. The two cDNAs are 85% identical at the nucleotide level. Similar to other proteins imported into mitochondria, the deduced human COX Vb protein contains a presequence, 31 amino acids long, rich in basic residues. We find no evidence for tissue-specific transcripts for subunit Vb of human COX, as Northern analysis of total RNA from human muscle, liver, and brain showed a single, identically sized transcript in each cell type, while partial-length cDNA clones isolated from human muscle and endothelial cell cDNA libraries were identical in sequence to the fetal muscle cDNA.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
DNA Restriction Enzymes
Genes
Humans
Macromolecular Substances
Mitochondria, Heart enzymology
Molecular Sequence Data
Nucleic Acid Hybridization
Nucleotide Mapping
Species Specificity
DNA genetics
Electron Transport Complex IV genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1119
- Volume :
- 65
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 2840351
- Full Text :
- https://doi.org/10.1016/0378-1119(88)90411-8