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Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension.
- Source :
-
Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2017 Aug; Vol. 71, pp. 55-62. Date of Electronic Publication: 2017 Apr 08. - Publication Year :
- 2017
-
Abstract
- We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only during pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine
Adult
Cytokinesis
Deoxyguanosine analogs & derivatives
Deoxyguanosine metabolism
Female
Humans
Lymphocytes drug effects
Lymphocytes metabolism
Methyldopa pharmacology
Methyldopa therapeutic use
Micronuclei, Chromosome-Defective
Micronucleus Tests
Pregnancy
Thiobarbituric Acid Reactive Substances metabolism
Young Adult
DNA Damage
Diabetes, Gestational genetics
Diabetes, Gestational metabolism
Hypertension, Pregnancy-Induced drug therapy
Hypertension, Pregnancy-Induced genetics
Hypertension, Pregnancy-Induced metabolism
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1873-1708
- Volume :
- 71
- Database :
- MEDLINE
- Journal :
- Reproductive toxicology (Elmsford, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 28400286
- Full Text :
- https://doi.org/10.1016/j.reprotox.2017.04.002