Back to Search
Start Over
Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2017 Apr 10; Vol. 22 (4). Date of Electronic Publication: 2017 Apr 10. - Publication Year :
- 2017
-
Abstract
- Dendrobium nobile Lindl., an epiphytic herb distributed in the Southeast Asia, is used as a tonic and antipyretic herbal medicine in China. In this study, a water-soluble acidic heteropolysaccharide, DNP-W4, containing mannose, glucose, galactose, xylose, rhamnose, and galacturonic acid, in the molar ratios of 1.0:4.9:2.5:0.5:1.0:0.9, was obtained from the stems of Dendrobium nobile Lindl. Using methylation analysis, partial acid hydrolysis, pectolyase treatment, NMR, and ESI-MS, the structure of DNP-W4 was elucidated. The obtained data indicated that DNP-W4 was a complex heteropolysaccharide and possessed a backbone composed of (1→4)-linked β-d-Glcp, (1→6)-linked β-d-Glcp, and (1→6)-linked β-d-Galp, with substitutes at O-4/6 of Glcp residues and O-3 of Galp. The branches of DNP-W4 were composed of terminal Manp, (1→6)-linked β-d-Manp, (1→3)-linked β-d-Glcp, β-d-Glcp, β-d-Galp, (1→4)-linked α-d-GalAp, (1→2)-linked α-L-Rhap, and Xylp. DNP-W4 had little immunological activities, but its derivatives had immuno-stimulating activities to some extent.
- Subjects :
- Animals
Immunologic Factors isolation & purification
Lymphocyte Activation drug effects
Lymphocytes drug effects
Lymphocytes immunology
Lymphocytes metabolism
Male
Mice
Plant Extracts isolation & purification
Polysaccharides isolation & purification
Spectrum Analysis
Structure-Activity Relationship
Dendrobium chemistry
Immunologic Factors chemistry
Immunologic Factors pharmacology
Plant Extracts chemistry
Plant Extracts pharmacology
Plant Stems chemistry
Polysaccharides chemistry
Polysaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 22
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 28394301
- Full Text :
- https://doi.org/10.3390/molecules22040611