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Proteomic analysis of human plasma and peripheral blood mononuclear cells in Systemic Lupus Erythematosus patients.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2017 Jul; Vol. 446, pp. 37-46. Date of Electronic Publication: 2017 Apr 05. - Publication Year :
- 2017
-
Abstract
- Systemic Lupus Erythematosus (SLE) is a systemic autoimmune disease with a broad spectrum of clinical presentations and incompletely understood pathogenesis. This autoimmune disease is characterized by alterations in both the innate and adaptive immune system that lead to the loss of immunologic tolerance. In autoimmune diseases particularly in SLE, early diagnosis, flare or remission phases can be difficult to identify. Proteomics can help to find new therapeutic targets and it also could help to better understand the cellular mechanisms. The aim of this study was to observe the variations in plasma and Peripheral Blood Mononuclear Cells (PBMCs) proteome in order to increase our knowledge about pathogenesis and to find possible diagnostic markers and/or therapeutic targets for improving diagnosis and treatment. The comparative proteomic analyses showed that several proteins were differentially expressed in the PBMCs from SLE patients. Among these, PRDX2 may be used as candidate biomarker or target protein for further investigations. In plasma, we showed that plasma clusterin levels increased in SLE patients compared to healthy controls, but this increase is not statistically significant. These proteomic results provide suggestions for understanding the molecular mechanisms of SLE, as well as the physiological changes correlated with SLE disease.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Adult
Aged
Biomarkers blood
Electrophoresis, Polyacrylamide Gel
Female
Flow Cytometry
Humans
Lupus Erythematosus, Systemic diagnosis
Lupus Erythematosus, Systemic immunology
Male
Middle Aged
Peroxiredoxins blood
Peroxiredoxins isolation & purification
Proteome analysis
Leukocytes, Mononuclear metabolism
Lupus Erythematosus, Systemic blood
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7905
- Volume :
- 446
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 28390925
- Full Text :
- https://doi.org/10.1016/j.jim.2017.03.019