Back to Search Start Over

BmajPLA 2 -II, a basic Lys49-phospholipase A 2 homologue from Bothrops marajoensis snake venom with parasiticidal potential.

Authors :
Grabner AN
Alfonso J
Kayano AM
Moreira-Dill LS
Dos Santos APA
Caldeira CAS
Sobrinho JC
Gómez A
Grabner FP
Cardoso FF
Zuliani JP
Fontes MRM
Pimenta DC
Gómez CV
Teles CBG
Soares AM
Calderon LA
Source :
International journal of biological macromolecules [Int J Biol Macromol] 2017 Sep; Vol. 102, pp. 571-581. Date of Electronic Publication: 2017 Apr 05.
Publication Year :
2017

Abstract

Snake venoms contain various proteins, especially phospholipases A <subscript>2</subscript> (PLA <subscript>2</subscript> s), which present potential applications in diverse areas of health and medicine. In this study, a new basic PLA <subscript>2</subscript> from Bothrops marajoensis with parasiticidal activity was purified and characterized biochemically and biologically. B. marajoensis venom was fractionated through cation exchange followed by reverse phase chromatographies. The isolated toxin, BmajPLA <subscript>2</subscript> -II, was structurally characterized with MALDI-TOF (Matrix-assisted laser desorption/ionization-time of flight) mass spectrometry, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by two-dimensional electrophoresis, partial amino acid sequencing, an enzymatic activity assay, circular dichroism, and dynamic light scattering assays. These structural characterization tests presented BmajPLA <subscript>2</subscript> -II as a basic Lys49 PLA <subscript>2</subscript> homologue, compatible with other basic snake venom PLA <subscript>2</subscript> s (svPLA <subscript>2</subscript> ), with a tendency to form aggregations. The in vitro anti-parasitic potential of B. marajoensis venom and of BmajPLA <subscript>2</subscript> -II was evaluated against Leishmania infantum promastigotes and Trypanosoma cruzi epimastigotes, showing significant activity at a concentration of 100μg/mL. The venom and BmajPLA <subscript>2</subscript> -II presented IC <subscript>50</subscript> of 0.14±0.08 and 6.41±0.64μg/mL, respectively, against intraerythrocytic forms of Plasmodium falciparum with CC <subscript>50</subscript> cytotoxicity values against HepG2 cells of 43.64±7.94 and >150μg/mL, respectively. The biotechnological potential of these substances in relation to leishmaniasis, Chagas disease and malaria should be more deeply investigated.<br /> (Copyright © 2017. Published by Elsevier B.V.)

Details

Language :
English
ISSN :
1879-0003
Volume :
102
Database :
MEDLINE
Journal :
International journal of biological macromolecules
Publication Type :
Academic Journal
Accession number :
28390830
Full Text :
https://doi.org/10.1016/j.ijbiomac.2017.04.013