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Identification of autoreactive B cells with labeled nucleosomes.
- Source :
-
Scientific reports [Sci Rep] 2017 Apr 04; Vol. 7 (1), pp. 602. Date of Electronic Publication: 2017 Apr 04. - Publication Year :
- 2017
-
Abstract
- The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype.
- Subjects :
- Animals
Autoantibodies immunology
Autoantigens immunology
Biomarkers
Cell Line
Female
Flow Cytometry
Humans
Lupus Erythematosus, Systemic immunology
Lupus Erythematosus, Systemic metabolism
Mice
Staining and Labeling
Autoimmunity
B-Lymphocytes immunology
B-Lymphocytes metabolism
Nucleosomes immunology
Nucleosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28377609
- Full Text :
- https://doi.org/10.1038/s41598-017-00664-0