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PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2017 May 24; Vol. 61 (6). Date of Electronic Publication: 2017 May 24 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- A PCR-dipstick chromatography technique was designed and evaluated for differential identification of bla <subscript>NDM</subscript> , bla <subscript>KPC</subscript> , bla <subscript>IMP</subscript> , and bla <subscript>OXA-48</subscript> carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.<br /> (Copyright © 2017 American Society for Microbiology.)
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 61
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 28373197
- Full Text :
- https://doi.org/10.1128/AAC.00067-17