PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.

Authors :: Shanmugakani RK
Akeda Y
Yamamoto N
Sakamoto N
Hagiya H
Yoshida H
Takeuchi D
Sugawara Y
Kodera T
Kawase M
Laolerd W
Chaihongsa N
Santanirand P
Ishii Y
Hamada S
Tomono K
Source :: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2017 May 24; Vol. 61 (6). Date of Electronic Publication: 2017 May 24 (Print Publication: 2017).
Publication Year :: 2017
Abstract: A PCR-dipstick chromatography technique was designed and evaluated for differential identification of bla <subscript>NDM</subscript> , bla <subscript>KPC</subscript> , bla <subscript>IMP</subscript> , and bla <subscript>OXA-48</subscript> carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.<br /> (Copyright © 2017 American Society for Microbiology.)

Subjects :: Carbapenems pharmacology
Humans
Bacterial Proteins genetics
Feces microbiology
Molecular Diagnostic Techniques methods
Multiplex Polymerase Chain Reaction methods
beta-Lactamases genetics

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