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Metazoan Nuclear Pores Provide a Scaffold for Poised Genes and Mediate Induced Enhancer-Promoter Contacts.
- Source :
-
Molecular cell [Mol Cell] 2017 Apr 06; Vol. 66 (1), pp. 63-76.e6. Date of Electronic Publication: 2017 Mar 30. - Publication Year :
- 2017
-
Abstract
- Nuclear pore complex components (Nups) have been implicated in transcriptional regulation, yet what regulatory steps are controlled by metazoan Nups remains unclear. We identified the presence of multiple Nups at promoters, enhancers, and insulators in the Drosophila genome. In line with this binding, we uncovered a functional role for Nup98 in mediating enhancer-promoter looping at ecdysone-inducible genes. These genes were found to be stably associated with nuclear pores before and after activation. Although changing levels of Nup98 disrupted enhancer-promoter contacts, it did not affect ongoing transcription but instead compromised subsequent transcriptional activation or transcriptional memory. In support of the enhancer-looping role, we found Nup98 to gain and retain physical interactions with architectural proteins upon stimulation with ecdysone. Together, our data identify Nups as a class of architectural proteins for enhancers and supports a model in which animal genomes use the nuclear pore as an organizing scaffold for inducible poised genes.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Animals, Genetically Modified
Binding Sites
Cell Line
Chromatin genetics
Drosophila Proteins genetics
Drosophila melanogaster drug effects
Drosophila melanogaster genetics
Ecdysone pharmacology
Genotype
Insulator Elements
Mutation
Nuclear Pore Complex Proteins genetics
Nuclear Pore Complex Proteins metabolism
Phenotype
Protein Binding
RNA Interference
Transfection
Chromatin metabolism
Drosophila Proteins metabolism
Drosophila melanogaster metabolism
Enhancer Elements, Genetic
Promoter Regions, Genetic
Transcription, Genetic drug effects
Transcriptional Activation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 66
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 28366641
- Full Text :
- https://doi.org/10.1016/j.molcel.2017.02.020