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Integration of Chemical, Genetic, and Bioinformatic Approaches Delineates Fungal Polyketide-Peptide Hybrid Biosynthesis.
- Source :
-
Organic letters [Org Lett] 2017 Apr 21; Vol. 19 (8), pp. 2002-2005. Date of Electronic Publication: 2017 Mar 31. - Publication Year :
- 2017
-
Abstract
- To identify natural products and their associated biosynthetic genes from underutilized, difficult-to-manipulate microbes, chemical screening and bioinformatic analysis were employed to identify secondary metabolites and a potentially associated biosynthetic gene cluster. Subsequently, a heterologous expression system was used to confirm the identity of the gene cluster and the proposed biosynthetic mechanism. This approach successfully identified the curvupallide and spirostaphylotrichin biosynthetic pathways in endophytic fungus Curvularia pallescens and the short-chain pyranonigrin biosynthetic pathway in Aspergillus niger.
- Subjects :
- Aspergillus nidulans genetics
Aspergillus nidulans metabolism
Aspergillus niger genetics
Aspergillus niger metabolism
Biological Products metabolism
Biosynthetic Pathways
Computational Biology
Genes, Fungal
Multigene Family
Peptides isolation & purification
Polyketides isolation & purification
Pyrones chemistry
Pyrones isolation & purification
Secondary Metabolism
Biological Products chemistry
Peptides chemistry
Polyketides chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1523-7052
- Volume :
- 19
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Organic letters
- Publication Type :
- Academic Journal
- Accession number :
- 28361537
- Full Text :
- https://doi.org/10.1021/acs.orglett.7b00559