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Probiotic bacteria prevent Salmonella - induced suppression of lymphoproliferation in mice by an immunomodulatory mechanism.
- Source :
-
BMC microbiology [BMC Microbiol] 2017 Mar 29; Vol. 17 (1), pp. 77. Date of Electronic Publication: 2017 Mar 29. - Publication Year :
- 2017
-
Abstract
- Background: Salmonella enterica infections often exhibit a form of immune evasion. We previously observed that probiotic bacteria could prevent inhibition of lymphoproliferation and apoptosis responses of T cells associated with S. enterica infections in orally challenged mice.<br />Results: In this study, changes in expression of genes related to lymphocyte activation in mucosa-associated lymphoid tissues (MALT) of mice orally infected with S. enterica with and without treatment with probiotic bacteria were evaluated. Probiotic bacteria increased expression of mRNA for clusters of differentiation antigen 2 (Cd2), protein tyrosine phosphatase receptor type C (Ptprc), and Toll-like receptor 6 (Tlr6) genes related to T and B cell activation in mouse intestinal tissue. The probiotic bacteria were also associated with reduced mRNA expression of a group of genes (RelB, Myd88, Iκκa, Jun, Irak2) related to nuclear factor of kappa light chains enhancer in B cells (NF-κB) signal transduction pathway-regulated cytokine responses. Probiotic bacteria were also associated with reduced mRNA expression of apoptotic genes (Casp2, Casp12, Dad1, Akt1, Bad) that suggest high avidity lymphocyte sparing. Reduced CD2 immunostaining in mesenteric lymph nodes (MLN) was suggestive of reduced lymphocyte activation in probiotic-treated mice. Reduced immunostaining of TLR6 in MALT of probiotic-treated, S. enterica-infected mice suggests that diminished innate immune sensitivity to S. enterica antigens is associated with preventing lymphocyte deletion.<br />Conclusions: The results of this study are consistent with prevention of S. enterica-induced deletion of lymphocytes by the influence of probiotic bacteria in mucosal lymphoid tissues of mice.
- Subjects :
- Animals
Apoptosis drug effects
Apoptosis genetics
B-Lymphocytes metabolism
CD2 Antigens biosynthesis
CD2 Antigens genetics
Cytokines genetics
Disease Models, Animal
Female
Gene Expression Regulation
Immunosuppression Therapy
Leukocyte Common Antigens biosynthesis
Leukocyte Common Antigens genetics
Lymph Nodes
Lymphocytes
Male
Mice
Mice, Inbred BALB C
NF-kappa B metabolism
Peyer's Patches drug effects
Peyer's Patches immunology
Peyer's Patches pathology
RNA, Messenger biosynthesis
Signal Transduction drug effects
Signal Transduction genetics
Spleen
Toll-Like Receptor 6 biosynthesis
Toll-Like Receptor 6 genetics
Cell Proliferation drug effects
Immunomodulation
Probiotics pharmacology
Salmonella Infections, Animal prevention & control
Salmonella enterica pathogenicity
T-Lymphocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2180
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 28356067
- Full Text :
- https://doi.org/10.1186/s12866-017-0990-x