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The impact of succinate trace on pWW0 and ortho-cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation.
- Source :
-
Bioresource technology [Bioresour Technol] 2017 Jun; Vol. 234, pp. 397-405. Date of Electronic Publication: 2017 Mar 16. - Publication Year :
- 2017
-
Abstract
- Toluene is a pollutant catabolised through the interconnected pWW0 (TOL) and ortho-cleavage pathways of Pseudomonas putida mt-2, while upon succinate and toluene mixtures introduction in batch cultures grown on M9 medium, succinate was previously reported as non-repressing. The effect of a 40 times lower succinate concentration, as compared to literature values, was explored through systematic real-time qPCR monitoring of transcriptional kinetics of the key TOL Pu, Pm and ortho-cleavage PbenR, PbenA promoters in mixed-substrate experiments. Even succinate trace inhibited transcription leading to bi-modal promoters expression. Potential carbon catabolite repression mechanisms and novel expression patterns of promoters were unfolded. Lag phase was shortened and biomass growth levels increased compared to sole toluene biodegradation suggesting enhanced pollutant removal efficiency. The study stressed the noticeable effect of a preferred compound's left-over on the main route of a bioprocess, revealing the beneficiary supply of low preferred substrates concentrations to design optimal bioremediation strategies.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Batch Cell Culture Techniques
Biodegradation, Environmental
Bioreactors
Gene Expression Regulation, Bacterial
Kinetics
Metabolic Networks and Pathways
Promoter Regions, Genetic
Pseudomonas putida genetics
Transcription, Genetic
Environmental Pollutants metabolism
Plasmids genetics
Pseudomonas putida metabolism
Succinates metabolism
Toluene metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 234
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 28347959
- Full Text :
- https://doi.org/10.1016/j.biortech.2017.03.082