New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.

Authors :: Naguib MM
El-Kady MF
Lüschow D
Hassan KE
Arafa AS
El-Zanaty A
Hassan MK
Hafez HM
Grund C
Harder TC
Source :: Journal of virological methods [J Virol Methods] 2017 Jul; Vol. 245, pp. 19-27. Date of Electronic Publication: 2017 Mar 21.
Publication Year :: 2017
Abstract: In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)

Full Text Access

Tools