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Chitin extraction from blue crab (Portunus segnis) and shrimp (Penaeus kerathurus) shells using digestive alkaline proteases from P. segnis viscera.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2017 Aug; Vol. 101, pp. 455-463. Date of Electronic Publication: 2017 Mar 20. - Publication Year :
- 2017
-
Abstract
- Since chitin is closely associated with proteins, deproteinization is a crucial step in the process of extracting chitin. Thus, this research aimed to extract chitin from Portunus segnis and Penaeus kerathurus shells by means of crude digestive alkaline proteases from the viscera of P. segnis, regarding deproteinization step, as an alternative to chemical treatment. Casein zymography revealed that five caseinolytic proteases bands exist, suggesting the presence of at least five different major proteases. The optimum pH and temperature for protease activity were pH 8.0 and 60°C, respectively, using casein as a substrate. The crude enzymes extract was highly stable at low temperatures and over a wide range of pH from 6.0 to 12.0. The crude alkaline protease extract was found to be effective in the deproteinization of blue crab and shrimp shells, to produce chitin. The best efficiency in deproteinization (84.69±0.65% for blue crab shells and 91.06±1.40% for shrimp shells) was achieved with an E/S ratio of 5U/mg of proteins after 3h incubation at 50°C. These results suggest that enzymatic deproteinization of crab and shrimp wastes by fish endogenous alkaline proteases could be a potential alternative in the chitin production process.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bacterial Proteins antagonists & inhibitors
Brachyura enzymology
Hydrogen-Ion Concentration
Metals pharmacology
Protease Inhibitors pharmacology
Temperature
Bacterial Proteins metabolism
Brachyura chemistry
Chitin isolation & purification
Digestion
Endopeptidases metabolism
Penaeidae chemistry
Viscera enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 101
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 28336276
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2017.02.103