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C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia.
- Source :
-
Brain : a journal of neurology [Brain] 2017 Apr 01; Vol. 140 (4), pp. 887-897. - Publication Year :
- 2017
-
Abstract
- A non-coding hexanucleotide repeat expansion in intron 1 of the C9orf72 gene is the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (C9ALS/FTD), however, the precise molecular mechanism by which the C9orf72 hexanucleotide repeat expansion directs C9ALS/FTD pathogenesis remains unclear. Here, we report a novel disease mechanism arising due to the interaction of C9ORF72 with the RAB7L1 GTPase to regulate vesicle trafficking. Endogenous interaction between C9ORF72 and RAB7L1 was confirmed in human SH-SY5Y neuroblastoma cells. The C9orf72 hexanucleotide repeat expansion led to haploinsufficiency resulting in severely defective intracellular and extracellular vesicle trafficking and a dysfunctional trans-Golgi network phenotype in patient-derived fibroblasts and induced pluripotent stem cell-derived motor neurons. Genetic ablation of RAB7L1or C9orf72 in SH-SY5Y cells recapitulated the findings in C9ALS/FTD fibroblasts and induced pluripotent stem cell neurons. When C9ORF72 was overexpressed or antisense oligonucleotides were targeted to the C9orf72 hexanucleotide repeat expansion to upregulate normal variant 1 transcript levels, the defective vesicle trafficking and dysfunctional trans-Golgi network phenotypes were reversed, suggesting that both loss- and gain-of-function mechanisms play a role in disease pathogenesis. In conclusion, we have identified a novel mechanism for C9ALS/FTD pathogenesis highlighting the molecular regulation of intracellular and extracellular vesicle trafficking as an important pathway in C9ALS/FTD pathogenesis.<br /> (© The Author (2017). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Amyotrophic Lateral Sclerosis genetics
Amyotrophic Lateral Sclerosis pathology
Animals
Biological Transport
C9orf72 Protein
COS Cells
Cell Line
Chlorocebus aethiops
DNA Repeat Expansion
Fibroblasts drug effects
Fibroblasts pathology
Frontotemporal Dementia genetics
Frontotemporal Dementia pathology
Humans
Introns
Motor Neurons drug effects
Motor Neurons pathology
Neurons drug effects
Neurons pathology
Oligonucleotides, Antisense pharmacology
Pedigree
Pluripotent Stem Cells drug effects
Pluripotent Stem Cells pathology
Proteins genetics
rab GTP-Binding Proteins
rab1 GTP-Binding Proteins genetics
Amyotrophic Lateral Sclerosis metabolism
Frontotemporal Dementia metabolism
Proteins metabolism
rab1 GTP-Binding Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2156
- Volume :
- 140
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Brain : a journal of neurology
- Publication Type :
- Academic Journal
- Accession number :
- 28334866
- Full Text :
- https://doi.org/10.1093/brain/awx024