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Bioassay-Guided Isolation of Anti-Inflammatory Components from the Bulbs of Lilium brownii var. viridulum and Identifying the Underlying Mechanism through Acting on the NF-κB/MAPKs Pathway.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2017 Mar 23; Vol. 22 (4). Date of Electronic Publication: 2017 Mar 23. - Publication Year :
- 2017
-
Abstract
- The bulbs of Lilium brownii var. viridulum (LB) are commonly used as both traditional Chinese medicines and popular functional food for many centuries in China. Previous studies reported that the extract of lily bulbs exhibited anti-inflammatory activity both in vivo and in vitro, but its active components and associated molecular mechanisms remain elusive. In the present study, using bioassay-guided isolation method, two phenylpropenoid acylglycerols, 1- O -feruloyl-2- O - p -coumaroylglycerol ( 1 ) and 1,3- O -diferuloylglycerol ( 2 ), were obtained and identified from the chloroform fraction of LB. Both compounds 1 and 2 significantly decreased the production of nitrite oxide (NO) in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells in a dose-dependent manner with half maximal inhibitory concentration (IC <subscript>50</subscript> ) values of 9.12 ± 0.72 μM and 12.01 ± 1.07 μM, respectively. They also inhibited the production of prostaglandin E2 (PGE2) and several other pro-inflammatory cytokines, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). Furthermore, compounds 1 and 2 downregulated the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). They also inhibited the nuclear translocation of nuclear factor-κB (NF-κB) p65 subunit and suppressed mitogen-activated protein kinases (MAPKs) pathway. Taken these data together, compounds 1 and 2 exhibited anti-inflammatory activities through acting on the NF-κB and MAPKs pathway. This research provides the first evidence on the major bioactive constituents and related molecular mechanisms of LB as an anti-inflammatory agent. Our findings also advanced the understanding of LB as a traditional herbal medicine for the prevention and treatment of inflammation.
- Subjects :
- Animals
Anti-Inflammatory Agents chemistry
Anti-Inflammatory Agents pharmacology
Dose-Response Relationship, Drug
Gene Expression Regulation drug effects
Lipopolysaccharides pharmacology
MAP Kinase Signaling System drug effects
Mice
Nitric Oxide metabolism
Plant Extracts chemistry
Plant Extracts pharmacology
RAW 264.7 Cells
Anti-Inflammatory Agents isolation & purification
Lilium chemistry
NF-kappa B metabolism
Plant Extracts isolation & purification
Plant Roots chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 22
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 28333094
- Full Text :
- https://doi.org/10.3390/molecules22040506