Variation in growth rate, carbon assimilation, and photosynthetic efficiency in response to nitrogen source and concentration in phytoplankton isolated from upper San Francisco Bay.

Six species of phytoplankton recently isolated from upper San Francisco Bay were tested for their sensitivity to growth inhibition by ammonium (NH ₄ ⁺ ), and for differences in growth rates according to inorganic nitrogen (N) growth source. The quantum yield of photosystem II (F _v /F _m ) was a sensitive indicator of NH ₄ ⁺ toxicity, manifested by a suppression of F _v /F _m in a dose-dependent manner. Two chlorophytes were the least sensitive to NH ₄ ⁺ inhibition, at concentrations of >3,000 μmoles NH ₄ ⁺  · L ^-1 , followed by two estuarine diatoms that were sensitive at concentrations >1,000 μmoles NH ₄ ⁺  · L ^-1 , followed lastly by two freshwater diatoms that were sensitive at concentrations between 200 and 500 μmoles NH ₄ ⁺  · L ^-1 . At non-inhibiting concentrations of NH ₄ ⁺ , the freshwater diatom species grew fastest, followed by the estuarine diatoms, while the chlorophytes grew slowest. Variations in growth rates with N source did not follow taxonomic divisions. Of the two chlorophytes, one grew significantly faster on nitrate (NO ₃ ^- ), whereas the other grew significantly faster on NH ₄ ⁺ . All four diatoms tested grew faster on NH ₄ ⁺ compared with NO ₃ ^- . We showed that in cases where growth rates were faster on NH ₄ ⁺ than they were on NO ₃ ^- , the difference was not larger for chlorophytes compared with diatoms. This holds true for comparisons across a number of culture investigations suggesting that diatoms as a group will not be at a competitive disadvantage under natural conditions when NH ₄ ⁺ dominates the total N pool and they will also not have a growth advantage when NO ₃ ^- is dominant, as long as N concentrations are sufficient.
(© 2017 The Authors Journal of Phycology published by Wiley Periodicals, Inc. on behalf of Phycological Society of America.)