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Ultrastructural study of morphological changes in Schistosoma mansoni after in vitro exposure to the monoterpene rotundifolone.
- Source :
-
Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2017 Jan-Feb; Vol. 50 (1), pp. 86-91. - Publication Year :
- 2017
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Abstract
- Introduction:: Schistosomiasis, a parasitic disease caused by trematode flatworms of the genus Schistosoma, affects more than 200 million people worldwide, and its control is dependent on a single drug, praziquantel. Here, we report the in vitro effect of rotundifolone, a monoterpene isolated from Mentha x villosa (Lamiaceae), on Schistosoma mansoni adult worms.<br />Methods:: The in vitro effect of rotundifolone on adult Schistosoma mansoni was evaluated by analysis of behavior and mortality and through a scanning electron microscopic analysis of ultrastructural changes in the tegument of the worms.<br />Results:: At concentrations of 3.54 and 7.09μg/mL-1 rotundifolone, no worm mortality was observed at any of the sampling intervals. A minor reduction in movement of the tail, suckers, and gynecophoral canal membrane was observed after 96 h of exposure to 7.09μg/mL-1 rotundifolone. At 70.96μg/mL-1, a lack of movement was observed from 72h onwards and all worms were deemed dead; similar effects were observed at 48h with 177.4μg/mL-1, and at 24h with 354.8μg/mL-1 and 700.96μg/mL-1. Rotundifolone also caused death of all parasites and separation of coupled pairs into individual males and females after 24h at 354.8μg/mL-1.<br />Conclusions:: The main changes in the tegument induced by the different ROT treatments were: after 24h incubation, bubble lesions spread over the entire body and loss of tubercles occurred in some regions of the ventral region.
Details
- Language :
- English
- ISSN :
- 1678-9849
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Revista da Sociedade Brasileira de Medicina Tropical
- Publication Type :
- Academic Journal
- Accession number :
- 28327807
- Full Text :
- https://doi.org/10.1590/0037-8682-0285-2016