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Effects of miR-21 downregulation and silibinin treatment in breast cancer cell lines.
- Source :
-
Cytotechnology [Cytotechnology] 2017 Aug; Vol. 69 (4), pp. 667-680. Date of Electronic Publication: 2017 Mar 20. - Publication Year :
- 2017
-
Abstract
- Silibinin is a natural polyphenol with high antioxidant and anticancer properties, which causes cell cycle arrest and apoptosis in most cancer cell types including breast cancer, but the in-line mechanisms, are still unknown. Silibinin significantly downregulated oncomiR miR-21 expression in breast cancer cells. Here the effect of anti-miR-21 on cell viability, apoptotic induction, cell cycle distribution, and the expression levels of downstream targets of miR-21 were investigated in MCF-7 and T47D cells. MiR-21 mimic transfection was also applied in silibinin treated samples to evaluate functional role of miR-21downregulation on silibinin effects. It was found that after anti-miR-21 transfection, no significant changes were detected in cell viability, apoptosis (except early apoptosis), and cell cycle in MCF-7 and T47D cells. Compared to silibinin, miR-21 mimic transfection in combination with silibinin caused a slight modulation in some of the examined silibinin effects including apoptosis, Bcl2 mRNA and PTEN mRNA and protein levels. Silibinin slightly changed luciferase activity from reporters containing the miR-21 recognition elements from PTEN-3'UTR and Bcl2-3'UTR in both cell lines. Together these data demonstrated negligible cancer-progression impact of miR-21 and limited roles of miR-21 downregulation in examined silibinin effects, and strengthened the anti-cancer pathways of silibinin other than miR-21downregulation in MCF-7 and T47D cells.
Details
- Language :
- English
- ISSN :
- 0920-9069
- Volume :
- 69
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cytotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 28321778
- Full Text :
- https://doi.org/10.1007/s10616-017-0076-5