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Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae.

Authors :
Dumke R
Benitez AJ
Chalker V
Gullsby K
Henrich B
Hidalgo-Grass C
Hoogenboezem T
Kese D
Loens K
Maaskant J
Michael-Gayego A
Moses AE
Nir-Paz R
Pas SD
Pereyre S
Petersen RF
Rosenblatt M
van Rossum AMC
Uldum SA
Unger WWJ
Ursi D
Winchell JM
Bebear C
Source :
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 Jun; Vol. 88 (2), pp. 111-114. Date of Electronic Publication: 2017 Mar 08.
Publication Year :
2017

Abstract

Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1879-0070
Volume :
88
Issue :
2
Database :
MEDLINE
Journal :
Diagnostic microbiology and infectious disease
Publication Type :
Academic Journal
Accession number :
28318608
Full Text :
https://doi.org/10.1016/j.diagmicrobio.2017.03.004