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Grape seed extract ameliorates bleomycin-induced mouse pulmonary fibrosis.
- Source :
-
Toxicology letters [Toxicol Lett] 2017 May 05; Vol. 273, pp. 1-9. Date of Electronic Publication: 2017 Mar 12. - Publication Year :
- 2017
-
Abstract
- Pulmonary fibrosis is common in a variety of inflammatory lung diseases, such as interstitial pneumonia, chronic obstructive pulmonary disease, and silicosis. There is currently no effective clinical drug treatment. It has been reported that grape seed extracts (GSE) has extensive pharmacological effects with minimal toxicity. Although it has been found that GSE can improve the lung collagen deposition and fibrosis pathology induced by bleomycin in rat, its effects on pulmonary function, inflammation, growth factors, matrix metalloproteinases and epithelial-mesenchymal transition remain to be researched. In the present study, we studied whether GSE provided protection against bleomycin (BLM)-induced mouse pulmonary fibrosis. ICR strain mice were treated with BLM in order to establish pulmonary fibrosis models. GSE was given daily via intragastric administration for three weeks starting at one day after intratracheal instillation. GSE at 50 or 100mg/kg significantly reduced BLM-induced inflammatory cells infiltration, proinflammatory factor protein expression, and hydroxyproline in lung tissues, and improved pulmonary function in mice. Additionally, treatment with GSE also significantly impaired BLM-induced increases in lung fibrotic marker expression (collagen type I alpha 1 and fibronectin 1) and decreases in an anti-fibrotic marker (E-cadherin). Further investigation indicated that the possible molecular targets of GSE are matrix metalloproteinases-9 (MMP-9) and TGF-β1, given that treatment with GSE significantly prevented BLM-induced increases in MMP-9 and TGF-β1 expression in the lungs. Together, these results suggest that supplementation with GSE may improve the quality of life of lung fibrosis patients by inhibiting MMP-9 and TGF-β1 expression in the lungs.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Anti-Inflammatory Agents administration & dosage
Bronchoalveolar Lavage Fluid chemistry
Female
Grape Seed Extract administration & dosage
Lung immunology
Lung pathology
Matrix Metalloproteinase 9 metabolism
Mice, Inbred ICR
Pulmonary Fibrosis chemically induced
Pulmonary Fibrosis immunology
Pulmonary Fibrosis pathology
Respiratory Function Tests
Transforming Growth Factor beta metabolism
Anti-Inflammatory Agents therapeutic use
Antibiotics, Antineoplastic toxicity
Bleomycin toxicity
Grape Seed Extract therapeutic use
Lung drug effects
Pulmonary Fibrosis prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 273
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 28300665
- Full Text :
- https://doi.org/10.1016/j.toxlet.2017.03.012