Back to Search
Start Over
Evaluation of seasonal influenza vaccines for H1N1pdm09 and type B viruses based on a replication-incompetent PB2-KO virus.
- Source :
-
Vaccine [Vaccine] 2017 Apr 04; Vol. 35 (15), pp. 1892-1897. Date of Electronic Publication: 2017 Mar 09. - Publication Year :
- 2017
-
Abstract
- Vaccination is the first line of protection against influenza virus infection in humans. Although inactivated and live-attenuated vaccines are available, each vaccine has drawbacks in terms of immunogenicity and safety. To overcome these issues, our group has developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that replicates only in PB2-expressing cells. Here we generated PB2-KO viruses possessing the hemagglutinin (HA) and neuraminidase (NA) segments from H1N1pdm09 or type B viruses and tested their vaccine potential. The two PB2-KO viruses propagated efficiently in PB2-expressing cells, and expressed chimeric HA as expected. Virus-specific IgG and IgA antibodies were detected in mice immunized with the viruses, and the immunized mice showed milder clinical signs and/or lower virus replication levels in the respiratory tract upon virus challenge. Our results indicate that these PB2-KO viruses have potential as vaccine candidates.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Viral blood
Disease Models, Animal
Female
Immunoglobulin A blood
Immunoglobulin G blood
Influenza A Virus, H1N1 Subtype genetics
Influenza B virus genetics
Influenza Vaccines administration & dosage
Influenza Vaccines genetics
Mice, Inbred BALB C
Orthomyxoviridae Infections pathology
Orthomyxoviridae Infections prevention & control
Respiratory System virology
Viral Load
Gene Knockout Techniques
Influenza A Virus, H1N1 Subtype immunology
Influenza B virus immunology
Influenza Vaccines immunology
Viral Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 35
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 28285982
- Full Text :
- https://doi.org/10.1016/j.vaccine.2017.02.041