Back to Search
Start Over
"Perfect" designer chromosome V and behavior of a ring derivative.
- Source :
-
Science (New York, N.Y.) [Science] 2017 Mar 10; Vol. 355 (6329). - Publication Year :
- 2017
-
Abstract
- Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the "Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.<br /> (Copyright © 2017, American Association for the Advancement of Science.)
- Subjects :
- Bacterial Proteins
CRISPR-Associated Protein 9
Chromosomes, Artificial, Yeast genetics
Clustered Regularly Interspaced Short Palindromic Repeats
Endonucleases
Gene Editing
Gene Rearrangement
Meiosis
Models, Genetic
Saccharomyces cerevisiae cytology
Transformation, Genetic
Chromosomes, Artificial, Yeast chemistry
Genome, Fungal
Saccharomyces cerevisiae genetics
Synthetic Biology methods
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 355
- Issue :
- 6329
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 28280151
- Full Text :
- https://doi.org/10.1126/science.aaf4704