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Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus.
- Source :
-
Clinics (Sao Paulo, Brazil) [Clinics (Sao Paulo)] 2017 Feb 01; Vol. 72 (2), pp. 111-115. Date of Electronic Publication: 2017 Feb 01. - Publication Year :
- 2017
-
Abstract
- Objectives:: The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus.<br />Methods:: Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method.<br />Results:: A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls.<br />Conclusion:: Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings.
Details
- Language :
- English
- ISSN :
- 1980-5322
- Volume :
- 72
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinics (Sao Paulo, Brazil)
- Publication Type :
- Academic Journal
- Accession number :
- 28273235
- Full Text :
- https://doi.org/10.6061/clinics/2017(02)08