Charge transfer chromatography used to study the interaction between synthetic phospholipids and nonylphenyl-nonylglycolate.

The interaction between 8 synthetic phospholipids and the nonionic tenzide nonylphenyl-nonylglycolate was studied by charge transfer chromatography. The method has been improved by carrying out the determination at different organic phase concentrations of eluent and by extrapolating the interactive strength back to pure water. It was suggested that two types of interaction of commensurable strength may exist between the tenzide and the phospholipids; hydrophil-hydrophil interactions between the polar head groups of phospholipids and the hydrophilic ethylene-oxide chain of tenzide; hydrophob-hydrophob interactions between the fatty acid chains of phospholipids and the alkyl chain of tenzide. This last effect strongly depends on the length of the fatty acid chain, but it is independent of the presence of double bonds in the lipophilic region of phospholipid.