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Hepatoprotective effect of Coreopsis tinctoria flowers against carbon tetrachloride-induced liver damage in mice.
- Source :
-
BMC complementary and alternative medicine [BMC Complement Altern Med] 2017 Mar 04; Vol. 17 (1), pp. 139. Date of Electronic Publication: 2017 Mar 04. - Publication Year :
- 2017
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Abstract
- Background: Coreopsis tinctoria is a traditional remedy for the management of various diseases including hepatitis. The hepatoprotective role of the plant is not scientifically explored till now. This study was designed to investigate the hepatoprotective potentials of the ethanol extract from C. tinctoria (CTEtOH) using an animal model of carbon tetrachloride (CCl <subscript>4</subscript> )-induced acute liver injury.<br />Methods: CTEtOH (0.5 and 1.0 g/kg) and silymarin (200 mg/kg) were administered to the experimental mice for 7 days followed by 0.2% CCl <subscript>4</subscript> (10 mL/kg of body weight (bw), ip), then all mice were sacrificed after 24 h. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured. Histological analysis of liver was performed. The tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), nitric oxide (NO), malondialdehyde (MDA), and antioxidant enzymatic activities were also measured..<br />Results: The results revealed that the serum ALT and AST levels significantly decreased after treatment with CTEtOH. Moreover, histological analyses indicated that CTEtOH (0.5 and 1.0 g/kg) and silymarin reduced the extent of CCl <subscript>4</subscript> -induced liver lesions. CTEtOH (0.5 and 1.0 g/kg) reduced the levels of malondialdehyde, nitric oxide, and proinflammatory cytokines (TNF-α and IL-1β). Furthermore, CTEtOH (1.0 g/kg) reduced the level of IL-6. The activities of antioxidant enzymes, namely superoxide dismutase and glutathione reductase, significantly increased after treatment with CTEtOH (0.5 and 1.0 g/kg) and that of glutathione peroxidase increased after treatment with 1.0 g/kg of CTEtOH.<br />Conclusions: These results demonstrate the hepatoprotective effect of CTEtOH against CCl <subscript>4</subscript> -induced acute liver injury in mice, and the underlying hepatoprotective mechanisms are associated with antioxidant and antiproinflammatory activities.
- Subjects :
- Animals
Cytokines
Liver pathology
Mice
Mice, Inbred ICR
Oxidoreductases drug effects
Oxidoreductases metabolism
Plant Extracts chemistry
Protective Agents chemistry
Carbon Tetrachloride toxicity
Chemical and Drug Induced Liver Injury pathology
Coreopsis chemistry
Flowers chemistry
Liver drug effects
Plant Extracts pharmacology
Protective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6882
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC complementary and alternative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 28259154
- Full Text :
- https://doi.org/10.1186/s12906-017-1604-8