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The effects of corticotrophin (ACTH1-24), cyclic AMP and TPA (12-O-tetradecanoyl phorbol-13-acetate) on DNA replication and proliferation of primary rabbit adrenocortical cells in a synthetic medium.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1987 Nov 13; Vol. 148 (3), pp. 1295-303. - Publication Year :
- 1987
-
Abstract
- ACTH1-24 stimulated the parenchymal cells in cultures of rat adrenal cortex in serum-free synthetic HiWoBa 2000 medium to replicate DNA, enter mitosis and divide. But ACTH's principal mediator, cyclic AMP, was not a complete mitogen: the adenylate cyclase-stimulating cholera toxin and dibutyryl cyclic AMP stimulated parenchymal cells to replicate DNA but not to enter mitosis. Thus, there must have been an additional mediator of the response to ACTH1-24 that enabled the parenchymal cells to enter mitosis. This additional mediator might have been protein kinase C because a protein kinase C activator and cyclic AMP elevator, TPA, stimulated the adrenocortical parenchymal cells to replicate DNA, enter mitosis and divide.
- Subjects :
- Animals
Bucladesine pharmacology
Cells, Cultured
Cholera Toxin pharmacology
Culture Media
Dibutyryl Cyclic GMP pharmacology
In Vitro Techniques
Rabbits
Adrenal Cortex cytology
Cell Division drug effects
Cosyntropin pharmacology
Cyclic AMP pharmacology
DNA Replication drug effects
Tetradecanoylphorbol Acetate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 148
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 2825683
- Full Text :
- https://doi.org/10.1016/s0006-291x(87)80274-7