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Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy.

Authors :
Rossi L
Nicoletti MC
Carmosino M
Mastrofrancesco L
Di Franco A
Indrio F
Lella R
Laviola L
Giorgino F
Svelto M
Gesualdo L
Procino G
Source :
Journal of diabetes research [J Diabetes Res] 2017; Vol. 2017, pp. 4360357. Date of Electronic Publication: 2017 Jan 26.
Publication Year :
2017

Abstract

Diabetic nephropathy (DN) is a microangiopathic complication of diabetes mellitus (DM) affecting one-third of diabetic patients. The large variability in the clinical presentation of renal involvement in patients with DM makes kidney biopsy a prerequisite for a correct diagnosis. However, renal biopsy is an invasive procedure associated with risk of major complications. Numerous studies aimed to identify a noninvasive biomarker of DN but, so far, none of these is considered to be sufficiently specific and sensitive. Water channel aquaporins (AQPs), expressed at the plasma membrane of epithelial tubular cells, are often dysregulated during DN. In this work, we analyzed the urine excretion of AQP5 and AQP2 (uAQP5 and uAQP2), via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM), 11 with proteinuric nondiabetic nephropathy (NDN), and 12 with histological diagnosis and classification of DN. ELISA and WB analysis independently showed that uAQP5 was significantly increased in DN patients. Interestingly, linear regression analysis showed a positive correlation between uAQP5 and the histological class of DN. The same analysis, focusing on uAQP2, showed comparable results. Taken together, these data suggest a possible use of AQP5 and AQP2 as novel noninvasive biomarkers to help in classifying the clinical stage of DN.<br />Competing Interests: The authors declare that they have no competing interests.

Details

Language :
English
ISSN :
2314-6753
Volume :
2017
Database :
MEDLINE
Journal :
Journal of diabetes research
Publication Type :
Academic Journal
Accession number :
28246612
Full Text :
https://doi.org/10.1155/2017/4360357