Back to Search
Start Over
Reduced interneuronal dendritic arborization in CA1 but not in CA3 region of mice subjected to chronic mild stress.
- Source :
-
Brain and behavior [Brain Behav] 2016 Dec 14; Vol. 7 (2), pp. e00534. Date of Electronic Publication: 2016 Dec 14 (Print Publication: 2017). - Publication Year :
- 2016
-
Abstract
- Introduction: Chronic stress induces dendritic atrophy and decreases spine density in excitatory hippocampal neurons, although there is also ample evidence indicating that the GABAergic system is altered in the hippocampus after this aversive experience. Chronic stress causes dendritic remodeling both in excitatory neurons and interneurons in the medial prefrontal cortex and the amygdala.<br />Methods: In order to know whether it also has an impact on the structure and neurotransmission of hippocampal interneurons, we have analyzed the dendritic arborization, spine density, and the expression of markers of inhibitory synapses and plasticity in the hippocampus of mice submitted to 21 days of mild restrain stress. The analyses were performed in GIN mice, a strain that displays EGFP-labeled interneurons.<br />Results: We observed a significant decrease in the dendritic arborization of interneurons in the CA1 region, which did not occur in those in CA3. We found neither changes in dendritic spine density in these regions nor alterations in the number of EGFP-positive interneurons. Nevertheless, the expression of glutamic acid decarboxylase 67 was reduced in different layers of CA1 and CA3 regions of the hippocampus. No significant changes were found in the expression of the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) or synaptophysin.<br />Conclusions: Chronic stress reduces the interneuronal dendritic arborization in CA1 region of the hippocampus but not in CA3.
- Subjects :
- Animals
Cell Count
Dendritic Spines enzymology
Interneurons cytology
Interneurons enzymology
Male
Mice
Neural Cell Adhesion Molecule L1 metabolism
Sialic Acids metabolism
CA1 Region, Hippocampal cytology
CA1 Region, Hippocampal enzymology
CA1 Region, Hippocampal physiopathology
CA3 Region, Hippocampal cytology
CA3 Region, Hippocampal enzymology
CA3 Region, Hippocampal physiopathology
Dendritic Spines physiology
Glutamate Decarboxylase metabolism
Interneurons physiology
Neuronal Plasticity physiology
Stress, Psychological enzymology
Stress, Psychological physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 2162-3279
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brain and behavior
- Publication Type :
- Academic Journal
- Accession number :
- 28239515
- Full Text :
- https://doi.org/10.1002/brb3.534