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Co-delivery of Doxorubicin Encapsulated PLGA Nanoparticles and Bcl-xL shRNA Using Alkyl-Modified PEI into Breast Cancer Cells.
- Source :
-
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2017 Sep; Vol. 183 (1), pp. 126-136. Date of Electronic Publication: 2017 Feb 24. - Publication Year :
- 2017
-
Abstract
- In recent years, much effort has been focused on an appropriate combination of chemotherapeutic drugs and nucleic acids to exploit additive or synergistic therapeutic effects and overcome many obstacles such as the reduction of side effects and drug resistance. Short hairpin RNA (shRNA) has designed to allow the production of small interfering RNA (siRNA) within the cells and offer long-lasting silencing of target genes. In this study, alkyl-modified polyethylenimine (PEI 10 kD) was used for co-delivery of doxorubicin (DOX) encapsulated into poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) and Bcl-xL shRNA (one class of molecules that block apoptosis of tumor cells) into breast cancer cells. Our results demonstrated that modification of PEI with alkyl chain could enhance the induction of apoptosis in tumor cells by suppression of Bcl-xL gene using Bcl-xL shRNA more than PEI alone. On the other hand, DOX encapsulated into PLGA had more synergistic effect with shRNA in comparison with DOX alone. In conclusion, combination of PLGA-DOX NPs and alkyl-PEI/shRNA complexes may have promising applications in breast cancer therapy.
- Subjects :
- Breast Neoplasms genetics
Breast Neoplasms metabolism
Female
Humans
MCF-7 Cells
Polylactic Acid-Polyglycolic Acid Copolymer
bcl-X Protein genetics
bcl-X Protein metabolism
Breast Neoplasms drug therapy
Drug Delivery Systems methods
Imines chemistry
Imines pharmacology
Lactic Acid chemistry
Lactic Acid pharmacology
Nanoparticles chemistry
Nanoparticles therapeutic use
Polyethylenes chemistry
Polyethylenes pharmacology
Polyglycolic Acid chemistry
Polyglycolic Acid pharmacology
RNA, Small Interfering chemistry
RNA, Small Interfering genetics
RNA, Small Interfering pharmacology
bcl-X Protein antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1559-0291
- Volume :
- 183
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Applied biochemistry and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 28236188
- Full Text :
- https://doi.org/10.1007/s12010-017-2434-3