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Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin.
- Source :
-
Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2017 Sep; Vol. 9 (3), pp. 334-344. - Publication Year :
- 2017
-
Abstract
- Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration-highly cytotoxic when applied at 25 μg/mL and no cytotoxicity at 5 μg/mL.
- Subjects :
- Biogenic Amines biosynthesis
Cell Line, Tumor
DNA, Bacterial isolation & purification
Enterococcus faecalis
Humans
Lactobacillus plantarum genetics
Listeria monocytogenes
Microbial Sensitivity Tests
Virulence Factors genetics
Bacteriocins biosynthesis
Drug Resistance, Multiple, Bacterial genetics
Genes, Bacterial
Lactobacillus plantarum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1867-1314
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Probiotics and antimicrobial proteins
- Publication Type :
- Academic Journal
- Accession number :
- 28233282
- Full Text :
- https://doi.org/10.1007/s12602-017-9260-3