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Isolation of Stromal Stem Cells from Adipose Tissue.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1553, pp. 169-182. - Publication Year :
- 2017
-
Abstract
- Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.
- Subjects :
- Animals
Biomarkers
Cell Culture Techniques
Cell Differentiation
Cell Lineage
Cells, Cultured
Cryopreservation methods
Humans
Immunophenotyping methods
Mice
Microscopy, Fluorescence
Adipose Tissue cytology
Cell Separation methods
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1553
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 28229415
- Full Text :
- https://doi.org/10.1007/978-1-4939-6756-8_13