Back to Search
Start Over
High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference.
- Source :
-
Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2017 May 01; Vol. 55 (6), pp. 817-825. - Publication Year :
- 2017
-
Abstract
- Background: High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS) and is expected to be adopted by a large number of patients. Biotin therapy leads to analytical interference in many immunoassays that utilize streptavidin-biotin capture techniques, yielding skewed results that can mimic various endocrine disorders. We aimed at exploring this interference, to be able to remove biotin and avoid misleading results.<br />Methods: We measured free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), parathyroid homrone (PTH), 25-hydroxyvitamin D (25OHD), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, C-peptide, cortisol (Roche Diagnostics assays), biotin and its main metabolites (liquid chromatography tandem mass spectrometry) in 23 plasmas from MS patients and healthy volunteers receiving high-dose biotin, and in 39 biotin-unsupplemented patients, before and after a simple procedure (designated N5) designed to remove biotin by means of streptavidin-coated microparticles. We also assayed fT4, TSH and PTH in the 23 high-biotin plasmas using assays not employing streptavidin-biotin binding.<br />Results: The biotin concentration ranged from 31.7 to 1160 µg/L in the 23 high-biotin plasmas samples. After the N5 protocol, the biotin concentration was below the detection limit in all but two samples (8.3 and 27.6 μg/L). Most hormones results were abnormal, but normalized after N5. All results with the alternative methods were normal except two slight PTH elevations. In the 39 biotin-unsupplemented patients, the N5 protocol did not affect the results for any of the hormones, apart from an 8.4% decrease in PTH.<br />Conclusions: We confirm that most streptavidin-biotin hormone immunoassays are affected by high biotin concentrations, leading to a risk of misdiagnosis. Our simple neutralization method efficiently suppresses biotin interference.
- Subjects :
- Biotin isolation & purification
Biotin metabolism
Case-Control Studies
Dose-Response Relationship, Drug
Female
Hormones blood
Humans
Male
Multiple Sclerosis blood
Multiple Sclerosis drug therapy
Streptavidin metabolism
Artifacts
Biotin therapeutic use
Blood Chemical Analysis methods
Endocrine System metabolism
Immunoassay methods
Subjects
Details
- Language :
- English
- ISSN :
- 1437-4331
- Volume :
- 55
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Clinical chemistry and laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 28222020
- Full Text :
- https://doi.org/10.1515/cclm-2016-1183