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Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection of Paramphistomum gracile circulating 16 kDa antigen.
- Source :
-
Parasitology [Parasitology] 2017 Jun; Vol. 144 (7), pp. 899-903. Date of Electronic Publication: 2017 Feb 21. - Publication Year :
- 2017
-
Abstract
- In this study, we have produced a monoclonal antibody (MoAb) against 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for the detection of circulating 16 kDaAg in the serum and fecal samples from cattle naturally infected with P. gracile. MoAb 1D10 was immobilized on a microtitre plate, and the antigen in the samples was captured and detected with biotinylated rabbit anti-16 kDaAgPg antibody. The lower detection limit of sandwich ELISA was 3·5 pg mL-1, and no cross-reaction with other parasite antigens was evaluated. The reliability of the assay was examined using the serum and fecal samples from cattle naturally infected with P. gracile, Fasciola gigantica, Moniezia benedeni, Trichuris sp., Strongyloides sp., strongylids and non-infected animals. The sandwich ELISA showed the sensitivity, specificity and accuracy at 98·33, 100 and 99·55% (serum samples), and 96·67, 100 and 99·09% (fecal samples). Therefore, this detection method is a rapid and excellent potential assay for the accurate diagnosis of paramphistomosis.
- Subjects :
- Animals
Antibodies, Helminth blood
Antibodies, Helminth immunology
Antibodies, Monoclonal blood
Antibodies, Monoclonal immunology
Cattle
Cattle Diseases blood
Cattle Diseases immunology
Cattle Diseases parasitology
Enzyme-Linked Immunosorbent Assay methods
Feces parasitology
Reproducibility of Results
Trematode Infections diagnosis
Trematode Infections immunology
Trematode Infections parasitology
Antigens, Helminth isolation & purification
Buffaloes
Cattle Diseases diagnosis
Enzyme-Linked Immunosorbent Assay veterinary
Immunologic Tests veterinary
Paramphistomatidae physiology
Trematode Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8161
- Volume :
- 144
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 28219472
- Full Text :
- https://doi.org/10.1017/S003118201600264X