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[Effect of the traditional Chinese medicine compound Yisui Lixue decoction on apoptosis of marrow cells in rats with myelodysplastic syndrome induced by dimethyl benzanthracene].
- Source :
-
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences [Zhong Nan Da Xue Xue Bao Yi Xue Ban] 2017 Jan 28; Vol. 42 (1), pp. 26-34. - Publication Year :
- 2017
-
Abstract
- Objective: To investigate the effect of the traditional Chinese medicine compound Yisui Lixue decoction on apoptosis of marrow cells in rats with myelodysplastic syndrome (MDS) induced by dimethyl benzanthracene (DMBA). Methods: The rats with MDS induced by the chemical mutagens DMBA were divided into 5 groups (12 rats in each group): a control group, a model+PBS group, a model+compound Zaofan pill group, a model+low dose of Yisui Lixue decoction group and a model+high dose of Yisui Lixue decoction group. After DMBA treatment for 14 days, rats were treated with different drugs for 1 month and executed on the 31 day. The peripheral blood cells, expressions of B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax), apoptosis of bone marrow cells were measured. Results: The counts of peripheral leukocyte, erythrocyte, hemoglobin and platelet in the model+PBS group were significantly decreased compared with those in the control group (P<0.01), while the peripheral hemograms of the treatment groups were improved significantly compared with those in the model+PBS group (P<0.05 or P<0.01), especially in the model+high dose group (P<0.01). In the model+PBS group, the expressions of Bax and Bcl-2 was significantly increased (P<0.01) or decreased (P<0.01), respectively, which were reversed by treatment of Yisui Lixue decoction (P<0.05 or P<0.01), especially in the model+high dose group (P<0.01). Compared with control group, the total apoptosis rate, earlier apoptosis and later apoptosis rate of bone marrow cells in the model+PBS group were increased (P<0.01), which were blocked by treatment of Yisui Lixue decoction (P<0.05 or P<0.01), and the model+high dose group was better than the model+low dose group. Conclusion: The traditional Chinese medicine compound Yisui Lixue decoction can improve peripheral hemogram, decrease the expression of Bax, increase the expression of Bcl-2, and reduce the apoptosis of bone marrow cells.
- Subjects :
- Animals
Benz(a)Anthracenes
Blood Cell Count
Bone Marrow
Gene Expression Regulation drug effects
Male
Medicine, Chinese Traditional
Myelodysplastic Syndromes chemically induced
Proto-Oncogene Proteins c-bcl-2 drug effects
Proto-Oncogene Proteins c-bcl-2 metabolism
Rats
bcl-2-Associated X Protein drug effects
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Bone Marrow Cells drug effects
Drugs, Chinese Herbal pharmacology
Drugs, Chinese Herbal therapeutic use
Myelodysplastic Syndromes drug therapy
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-7347
- Volume :
- 42
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28216494
- Full Text :
- https://doi.org/10.11817/j.issn.1672-7347.2017.01.005