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Evaluation of a field test for antibodies against Chlamydia trachomatis during trachoma surveillance in Nepal.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 May; Vol. 88 (1), pp. 3-6. Date of Electronic Publication: 2017 Jan 17. - Publication Year :
- 2017
-
Abstract
- Purpose: Testing for antibodies to Chlamydia trachomatis has potential as a surveillance tool. Our evaluation compares lateral flow assays (LFAs) during surveillance surveys in Nepal with Multiplex bead array (MBA). Fifty children were randomly sampled from each of 15 random clusters in two districts of Nepal. Finger prick blood samples were collected from 1509 children and tested onsite for anti-Pgp3 antibodies by LFA. The LFA was read at 30min as negative, positive, or invalid. Tests results were also rated as difficult to read ("equivocal"). Blood was processed at Johns Hopkins University using the MBA.<br />Results: The LFA had agreement of 40.0% for MBA-positive samples and 99.3% for MBA-negative samples. Inter-reader reliability was kappa=0.65 (95% CI=0.56-0.74). If the equivocal results (7%) could be decreased, reliability could be improved.<br />Conclusions: Further optimization and testing of the LFA test are needed to improve agreement with MBA and the interpretation of the results.<br /> (Copyright © 2017. Published by Elsevier Inc.)
- Subjects :
- Antigens, Bacterial immunology
Bacterial Proteins immunology
Child
Child, Preschool
Epidemiological Monitoring
Female
Humans
Infant
Male
Nepal epidemiology
Trachoma epidemiology
Antibodies, Bacterial blood
Chlamydia trachomatis immunology
Chromatography, Affinity methods
Immunomagnetic Separation methods
Serologic Tests methods
Trachoma diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 88
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 28214223
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2017.01.004