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Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.
- Source :
-
Journal of cell science [J Cell Sci] 2017 Apr 01; Vol. 130 (7), pp. 1217-1223. Date of Electronic Publication: 2017 Feb 16. - Publication Year :
- 2017
-
Abstract
- The immune synapse (IS) is a specialized structure formed at the contact area between T lymphocytes and antigen-presenting cells (APCs) that is essential for the adaptive immune response. Proper T cell activation requires its polarization towards the APC, which is highly dependent on the tubulin cytoskeleton. Microtubule-associated protein-4 (MAP4) is a microtubule (MT)-stabilizing protein that controls MTs in physiological processes, such as cell division, migration, vesicular transport or primary cilia formation. In this study, we assessed the role of MAP4 in T cell activation. MAP4 decorates the pericentrosomal area and MTs of the T cell, and it is involved in MT detyrosination and stable assembly in response to T cell activation. In addition, MAP4 prompts the timely translocation of the MT-organizing center (MTOC) towards the IS and the dynamics of signaling nanovesicles that sustains T cell activation. However, MAP4 acts as a negative regulator of other T cell activation-related signals, including diacylglycerol (DAG) production and IL2 secretion. Our data indicate that MAP4 acts as a checkpoint molecule that balances positive and negative hallmarks of T cell activation.<br /> (© 2017. Published by The Company of Biologists Ltd.)
- Subjects :
- Biomarkers metabolism
Diglycerides metabolism
Humans
Immunological Synapses metabolism
Jurkat Cells
Microtubule-Organizing Center metabolism
Receptors, Antigen, T-Cell metabolism
Signal Transduction
Lymphocyte Activation immunology
Microtubule-Associated Proteins metabolism
Microtubules metabolism
Nanoparticles chemistry
T-Lymphocytes immunology
Transport Vesicles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9137
- Volume :
- 130
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 28209780
- Full Text :
- https://doi.org/10.1242/jcs.199042