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Redox Proteomics and Platelet Activation: Understanding the Redox Proteome to Improve Platelet Quality for Transfusion.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Feb 11; Vol. 18 (2). Date of Electronic Publication: 2017 Feb 11. - Publication Year :
- 2017
-
Abstract
- Blood banks use pathogen inactivation (PI) technologies to increase the safety of platelet concentrates (PCs). The characteristics of PI-treated PCs slightly differ from those of untreated PCs, but the underlying reasons are not well understood. One possible cause is the generation of oxidative stress during the PI process. This is of great interest since reactive oxygen species (ROS) act as second messengers in platelet functions. Furthermore, there are links between protein oxidation and phosphorylation, another mechanism that is critical for cell regulation. Current research efforts focus on understanding the underlying mechanisms and identifying new target proteins. Proteomics technologies represent powerful tools for investigating signaling pathways involving ROS and post-translational modifications such as phosphorylation, while quantitative techniques enable the comparison of the platelet resting state versus the stimulated state. In particular, redox cysteine is a key player in platelet activation upon stimulation by different agonists. This review highlights the experiments that have provided insights into the roles of ROS in platelet function and the implications for platelet transfusion, and potentially in diseases such as inflammation and platelet hyperactivity. The review also describes the implication of redox mechanism in platelet storage considerations.
- Subjects :
- Animals
Antioxidants metabolism
Blood Preservation
Cysteine metabolism
Humans
NADPH Oxidases metabolism
Oxidative Stress
Phosphorylation
Platelet Aggregation
Platelet Transfusion
Reactive Oxygen Species metabolism
Signal Transduction
Blood Platelets metabolism
Oxidation-Reduction
Platelet Activation
Proteome
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28208668
- Full Text :
- https://doi.org/10.3390/ijms18020387