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Molecular characterization of a fish-specific toll-like receptor 22 (TLR22) gene from common carp (Cyprinus carpio L.): Evolutionary relationship and induced expression upon immune stimulants.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2017 Apr; Vol. 63, pp. 74-86. Date of Electronic Publication: 2017 Feb 11. - Publication Year :
- 2017
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Abstract
- In the host innate immune system, various pattern recognition receptors (PRRs) recognize conserved pathogens-associated molecular patterns (PAMPs), and represent an efficient first line of defense against invading pathogens. TLR22 is one of the fish-specific Toll-like receptors (TLRs), identified in a variety of fish species. In this study, we report the cloning and identification of a TLR22 cDNA from the gills of common carp (Cyprinus carpio L.). The full-length CcTLR22 cDNA was 3301 bp long, including a 32 bp 5'-untranslated region (UTR), an open reading frame (ORF) of 2838 bp and a 432 bp 3'-UTR.The CcTLR22 protein was found to comprise a signal peptide, 16 LRR domains, a LRRCT domain in the extracellular region and a TIR domain in the cytoplasmic region, which fits with the characteristic TLR domain architecture. The genomic organization of CcTLR22 was identified, which was encoded by an uninterrupted exon. Sequence alignment and phylogenetic analysis showed that all known teleost TLR22 members were clustered into an independent clade of the TLR22 family, and showed high amino acid identities with other fish TLRs. Real-time PCR assay showed that CcTLR22 mRNA was expressed in almost all tissues examined, while the levels obviously varied among different tissues. When challenged with poly(I:C) (a viral model) or A. hydrophila bacteria, the expression level of CcTLR22 was up-regulated in a variety of common carp tissues. These results indicate that CcTLR22 plays a significant role in systemic as well as mucosal defence after viral or bacterial stimulation or infection.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adjuvants, Immunologic pharmacology
Aeromonas hydrophila immunology
Amino Acid Sequence
Animals
DNA, Complementary genetics
DNA, Complementary metabolism
Fish Diseases microbiology
Fish Proteins chemistry
Fish Proteins genetics
Fish Proteins metabolism
Gram-Negative Bacterial Infections genetics
Gram-Negative Bacterial Infections immunology
Gram-Negative Bacterial Infections microbiology
Immunity, Innate drug effects
Immunity, Innate genetics
Organ Specificity
Phylogeny
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment veterinary
Toll-Like Receptors chemistry
Toll-Like Receptors metabolism
Carps classification
Carps genetics
Carps immunology
Evolution, Molecular
Fish Diseases immunology
Gene Expression Regulation drug effects
Gram-Negative Bacterial Infections veterinary
Poly I-C pharmacology
Toll-Like Receptors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 63
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 28192255
- Full Text :
- https://doi.org/10.1016/j.fsi.2017.02.009